The progression of telomerase activity and sialic acid levels in rat glioma cell lines

Abstract

Objective: Telomerase plays an important role in the cellular immortalization of cancers. It contains telomerase RNA, telomerase reverse transcriptase. Sialic acid consolidates the cellular membrane and plays a role in the maintenance of intercellular activity. Telomerase activity and sialic acid levels may be used to predict the biological behavior of C6 rat glioma cell lines. Materials and Methods: Telomerase activity and sialic acid levels were investigated in consecutive cell line pas¬sages in cell extracts taken from C6 rat glioma cell line. Both telomerase activity and sialic acid levels were deter¬mined in the 3rd, 6lh, 9, 12th and 15th passages. To detect telomerase activity, cell lysate was extracted by lysis buffer, and telomeric repeat amplification protocol was performed, using a PCR-based TRAP TeloTAGGG Te¬lomerase PCR ELISA PLUS plus kit. To examine sialic acid levels, a Sialic Acid Quantification Kit was used in the same passages that had been used for measurements of te¬lomerase activity. Results: Telomerase activity was found to be increased in the later passages of C6 cells, although it demonstrated a fluctuating pattern. Sialic acid levels were also seen to be increased, showing regular rising in the consecutive pas-sages of C6 cell line. Conclusion: In the consecutive passages of C6 cell line, an increase in sialic acid levels may tend to be regular, but an increase in telomerase activity' may be irregular. During the follow-up of glioma cell line, sialic acid levels may represent a more reliable biologic prognostic factor.

Amaç: Telorneraz kanser hiicrelcri nin o lum ­ siizltlgundc onemli bir rol oynar. Telomeraz. telorncraz RNA'sl ve tclomeraz revers transkriptazdan olusur. Siyalik asit, hucresel mcmbram guclendirir vc hucre ler arasi aktivitede bilyuk rol oynar. Polisiyalik asit zincirleri. noronal hucre adezyo n molckillunun adeziv fonksiyonunu dcg isririr. Telomeraz aktivitesi vc siyalik asit duzeylcri tumor belirleyicisi olarak kullamlabilir. Gerec pe Yiintem: C6 srcan glioma hucre dizisinden ard arda yapilan 3.. 6., 9., 12. ve 15. pasajla rm ekstraktlan nda, tclomeraz aktivitesi ve siyalik asit duzeyleri incelendi. Telomcraz aktivitesini belirlemek icin, TRAP TeloTAGGG Tclomerase PCR ELISA plus. siyalik asit duzeylerini belirlcmck icin, Sialic acid quantification kiti, kullamldi. Bulgulur: Te lomeraz aktivitesi C6 hucre dizisinin pasajlarmda, dalga lanmala r seklinde bir arus gosterdi, Tclorneraz RNA'sl ve telomeraz revers transkriptazm ekspresyonlan Him pasajlarda pozitif olarak izlendi. Siya lik asit duzeyleri, ilk pasajdan itibaren son pasaja dogru diizenli bir aI1 1~ gosterdi .

Sonuc: C6 hiicre dizilerinin ilerleyen pasajlan nda siyalik

asitin diizenli bir seki lde giderck artmasi, telo me raz aktivitcsindeki arusm ise duzcnsiz olmasi, gliomalan n klinik vc biyo lojik davr amslanrun be lirlenmesinde, tclorneraz aktivitesindcn ziyade, siyalik asitin daha guveni lir biyoloj ik prognostik faktor olabilecegin i dusundurmcktedir.