Bazı metallerin Pleurotus türlerinin boya dekolorizasyonu ve lakkaz, MnP enzim aktiviteleri üzerine olan etkisi

Abstract

This study searches the influence of distinct metals (HgCl2, Pb(NO3)2, Ni(NO3)2.6H2O, ZnSO4.7H2O, CoCl2.6H2O, Cd(NO3)2.4H2O, CuSO4.5H2O) on decolorization and extracelluler enzym activities by using Pleurotus ostreatus, Pleurotus sajor-caju ve Pleurotus florida. The study has found the inhibition of P. sajor caju’s development statistically meaningful (p≤0.05) in Pb and Ni’s 0,001 mM≤; Hg, Pb and Cd’s 0.005 mM≤ and Co’s 0.05 mM≤ concentrations. In side of P. florida, while 0.001 mM≤ concentration in all tested metals except for Ni an Cd is significant, the inhibition in Ni’s 0.005 mM≤ and Cd’s 0.01 mM≤ concentrations has been found statistically meaningful. In P. ostreatus, the inhibition has been determined statistically significant following Hg and Pb 0,01 mM ≤ concentration. Inhibition of development in 0,001 mM ≤ concentration has been found considerable in other metals. Enzym assays showed that metals did not make a significant change in enzyme activity of laccase. In P. sajor-caju all metal concentrations which were 20% inhibition have increased MnP activities compared with control groups In Pleurotus florida, Co 42% has increased MnP activity which is followed by Pb with 32%. MnP activities of Pleurotus ostreatus were increased with Hg and Ni but were decreased with Zn and Cd.