Antibiotic Resistance Of Coagulase Negative Staphylococci Isolated From Buffalo Milk And Some Milk Products

Abstract

The aim of this study was to determine the antibiotic resistance of coagulase- negative staphylococci (CNS) isolated from raw buffalo milk, tulum cheese and clotted cream in Afyonkarahisar province of Western Turkey. A total of 210 samples were examined between February 2008 and August 2009; of the samples examined 70 were raw buffalo milk, 70 tulum cheeses, and 70 clotted creams. Thirty four isolates (16.2%) obtained from 210 samples were detected as CNS. Of these isolates 12 (35.3%) were recovered from raw buffalo milk, 14 (41.2%) from tulum cheese and 8 (23.5%) from clotted cream. Kirby-Bauer disc diffusion method was used to detect the antibiotic resistance of CNS isolated from samples according to the standards of Clinical Laboratory Standards Institute. Erythromycin was found to be the least effective antibiotic as 79.4% of CNS isolates recovered from raw buffalo milk, tulum cheese and clotted cream samples were resistant to this drug, followed by amikacin (50%), cefalotine (44.1%), penicillin (38.2%), neomycin (35.3%), streptomycin (35.3%), gentamicin (32.4%) and oxacillin

Bu araştırmanın amacı, Türkiye’nin Bati illerinden olan Afyonkarahisar’da kaymak, tulum peyniri ve manda sütünden izole edilen koagulaz negatif stafilokokların antibiyotik direncinin belirlenmesidir. Şubat 2008 ile Ağustos 2009 döneminde 70’i tulum peyniri, 70’i kaymak, 70’i manda sütü olmak üzere toplam 210 örnek incelenmiştir. İncelenen 210 örneğin 34’ünden (%16.2) koagulaz negatif stafilokok izolati elde edilmiştir. Bu izolatlann 12’si (%35.3) manda sütünden, 14’ü (%41.2) tulum peynirinden, 8’i (%23.5) kaymaktan izole edilmiştir. Örneklerden izole edilen koagulaz negatif stafîlokoklann antibiyotik dirençliliğini belirlemek için CLSI’nin (Clinical Laboratory Standarts Institute) önerdiği Kirby-Bauer Disk Difüzyon metodu kullanıldı. Manda sütü, tulum peyniri ve kaymak örneklerinden elde edilen izolatlann %79.4’üne en az etkili antibiyotiğin eritromisin olduğu bulundu. Bunu %50 ile amikasin, %44.1 ile sefalotin, %38.2 ile penisilin, %35.3 ile streptomisin,