Manuel yöntem ile idrar sediment analizinin otomatik idrar analizöru ile kıyaslanması

Abstract

UF-100 analizoru eritrosit, lokosit, skuamoz ve transitional epitelial hticreleri, renal tubuler hticreleri, bakteri, hiyalen ve inklizyonal silendirleri, maya hticrelerini, kristalleri ve spermatozoalan argon lazer flow cytometry teknigi kullanarak ayirt eder. Biz bu cahsmada yaptigmnz mikroskopik idrar sediment analizi ile UF -100 'un sonuclanru karsilastirdik. Karstlastirmanm sonucunda lineer bir korelasyon saptandi, Eritrosit icin korelasyon katsayisi r = 0.88 iken Iokosit icin r == 0.91, epitel hiicresi icin ise r ==0.85 bulundu. Kristaller incelendiginde ise duyarlihk %71.4, ozgulluk %88.8 bulundu. Sonuc olarak UF-100 analizoru mikroskopide incelenmesi gereken numune sayisuu azaltarak, idrar analizi sonuclannm cikisuu hizlandmp is akisina kolayhk getirebilir.

The UF-100 analyser identifies erythrocyte, leukocyte, squamous epithelial cells, transitional epithelial and renal tubuler cell, bacteria, hyaline and inclusional casts, yeast-like cells, crystals and spermatazoa, by using argon laser flow cytometry technique. In this study we compared UF-100 analyser with visual microscopy for sediment counts. Comparison of results obtained by visual microscopy and by the UF-100 analyser showed a linear correlation with r=0.88 for erythrocyte, r = 0.91 for leuokocytes and r = 0.85 for epithelial cells. The analyser detected crystals with a sensitivity of 71.4 % and a specificity of 88.8 0/0. In conclusion, the UF­ 100 analyser can improve the work flow, increasing the output of urinalysis by reducing the number of specimens submitted for microscopy.