AKÜ Araştırma ve Akademik Performans Sistemi
DSpace@AKÜ, Afyon Kocatepe Üniversitesi’nin bilimsel araştırma ve akademik performansını izleme, analiz etme ve raporlama süreçlerini tek çatı altında buluşturan bütünleşik bilgi sistemidir.
Güncel Gönderiler
Öğe Türü: Öğe , Investigation of the effects of polydatin on gentamicin-induced renal toxicity in rats(Afyon Kocatepe Üniversitesi, 03.10.2025) Kandemir, Özge; Şimşek, Hasan; Akaras, Nurhan; Kandemir, Fatih MehmetGentamicin (GNT), an aminoglycoside antibiotic, induces nephrotoxicity through mechanisms like tubular apoptosis and inflammation. Polydatin (Poly), a natural polyphenolic compound with antioxidant and anti-inflammatory properties, has shown potential in alleviating renal damage. This study aimed to investigate the protective effects of Poly in rats with GNTinduced kidney injury using biochemical, molecular, and histopathological methods. 35 Wistar albino rats were divided into 5 groups (7 rats/group), including control, Poly (100 mg/kg), GNT (100 mg/kg), and two combined treatment groups (GNT+Poly at 50 mg/kg and 100 mg/kg). After 7 days of treatment, kidney tissues and blood were collected for analysis of renal function markers, oxidant-antioxidant parameters, gene expression (NF-κB, TNF-α, Caspase-3, Bax, Bcl-2, KIM1, AQP2), and histopathological evaluation. GNT increased serum urea and creatinine levels (p<0.001), increased MDA levels (p<0.001) and decreased antioxidants (p<0.001); also increased the expression of NF-κB and TNF-α (p<0.001), increased Caspase-3 and Bax (p<0.001) and decreased Bcl-2 levels (p<0.001). When administered together with GNT, Poly decreased MDA levels (p<0.001) and increased GSH levels (p<0.001), decreased inflammation markers (NF-κB and TNF-α) (p<0.01), decreased Caspase-3 and Bax (p<0.01) and increased Bcl-2 levels (p<0.01), and also improved histological damage and decreased histological score (p<0.05). In GNT-induced renal toxicity, Poly 100 treatment provided renal protection by reversing oxidative stress, inflammation, and apoptosis.Öğe Türü: Öğe , Determination of systemic inflammation and diagnostic markers using iron parameters in foals infected with rhodococcus equi(Afyon Kocatepe Üniversitesi, 03.10.2025) Deniz, Ömer; Atak, BedihaRhodococcus equi is a significant respiratory pathogen in foals, particularly those under six months of age. This study aimed to evaluate the effects of R. equi infection on serum iron (Fe), unsaturated iron-binding capacity (UIBC), and fibrinogen (Fbg) concentrations. Fifteen foals with confirmed R. equi pneumonia and ten healthy control foals were included. Infected foals were treated with oral azithromycin and rifampicin for 15 days. Samples were collected on days 0, 7, and 14, and Fe, UIBC, and Fbg levels were analyzed. Statistical analysis showed significantly higher UIBC and Fbg levels in the infected group at the start of treatment compared to controls (p=0.004 and p<0.001, respectively). Serum Fe levels did not differ significantly between groups (p=0.138), though a trend toward lower levels in infected foals was noted, reflecting the inflammatory response. Changes in UIBC and Fbg levels reflect the progression of infection and response to treatment. Among the parameters evaluated, fibrinogen appears to be the most reliable biomarker for monitoring systemic inflammation during R. equi pneumonia in foals. However, the diagnostic value of iron parameters warrants further investigation with larger sample sizes and time-point-specific analyses.Öğe Türü: Öğe , Büyük ruminantlarda akılcı olmayan antibiyotik kullanımının yaygınlığının araştırılması(Afyon Kocatepe Üniversitesi, 03.10.2025) Tüfekçi, Emre; Ural, Mert Okan; Karaahmetoğlu, Mehmet; Şahin, Orhan GaziBu çalışmada Kayseri ilindeki büyük ruminantlarda akılcı olmayan antibiyotik kullanımının yaygınlığının araştırılması amaçlandı. Bu amaçla Erciyes Üniversitesi Veteriner Fakültesi İç Hastalıkları Anabilim Dalı Ruminant Kliniği’ne teşhis ve tedavi amacıyla hayvanını getiren, daha önce antibiyotik tedavisi uygulamış 120 hayvan sahibine yüz-yüze anket çalışması yapıldı. Çalışmada, veteriner hekim tarafından muayene sonrası reçete edilen antibiyotik uygulamasına devam eden bilinçli hayvan sahipleri (Grup 1, n=23), veteriner hekim tarafından muayene edilmeden, verilen anemnez sonucu veteriner hekim tavsiyesi ile antibiyotik uygulaması yapan hayvan sahipleri (Grup 2, n=56) ve daha önceki uygulamalardan artan ve elinde bulunan antibiyotikleri rastgele seçerek uygulayan hayvan sahipleri (Grup 3, n=41) olarak gruplar oluşturuldu. Veteriner hekimlerin en çok kullandığı (%26,8) ve tavsiye ettiği (%37,0) antibiyotik grubunun beta laktam grubu olduğu tespit edildi. Benzer şekilde hayvan sahiplerinin de en çok kullandığı antibiyotik grubu beta laktam grubu antibiyotiklerdi (%30,8). Antibiyotik kullanılan hayvanlara farklı antibiyotik gruplarının tek veya kombine olarak uygulandığı tespit edildi. Tek bir antibiyotik kullanımının en çok veteriner hekim tarafından uyguladığı (%52,17) ve tavsiye edildiği (%60,71), ikili kombine antibiyotik kullanımının ise en çok Grup 3’teki katılımcılar tarafından uygulandığı görüldü (%48,78). İkiden daha fazla kombine antibiyotik kullanımının ise veteriner hekim tarafından uygulandığı (%21,74) belirlendi. Bu çalışma ile belirli bir ölçekte yaygın ve kontrol dışı antibiyotik kullanımı ortaya konuldu. Sonuç olarak gelecekte ortaya çıkması muhtemel bir gıda krizine karşı hayvansal üretimi korumak ve insan, hayvan ve çevre sağlığını olumsuz etkileyecek bilinçsiz antibiyotik kullanımı sonucu ortaya çıkabilecek dirençli suşlara karşı önlem almak, Veteriner hekimlerin ve yetiştiricilerin akılcı antibiyotik kullanımı hakkında bilgilendirmek, ulusal ve uluslararası boyutta önem arz etmektedir.Öğe Türü: Öğe , The effect of thyme oil and thymol on hepatic gene expression levels in rabbits with ınduced hepatic lipidosis(Afyon Kocatepe Üniversitesi, 03.10.2025) Ekinci, Gencay; Güneş, VehbiRabbits fed a high cholesterol diet (HCD) exhibit various physiopathologic features of hepatic lipidosis. The aim of this study was to investigate the effects of thyme oil and thymol on inflammation-related hepatic gene expression levels in a HCD-induced hepatic lipidosis model. Male New Zealand rabbits were divided into 6 groups. These groups were; Standard rabbit chow, Standard rabbit chow + thymol, Standard rabbit chow + thyme oil, High cholesterol diet, High cholesterol diet + thymol, High cholesterol diet + thyme oil. Blood samples were collected at 0, 4, 8 and 11 weeks during the study. Total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and triglyceride levels were analyzed. The mRNA expression levels of inflammation-related genes from liver tissue were analyzed by real-time polymerase chain reaction method. The expression levels of interleukin-4 (IL-4), IL-17 and interferon gamma genes were lower, whereas the expression levels of IL-9, IL-13, IL-18 and RAR-related orphan receptor gamma genes were higher in rabbits fed with HCD compared to the normal diet group (p<0.05). Thymol increased T-box transcription factor, IL-4, IL-17 and IL-18 gene expression. Thyme oil increased GATA binding protein 3 and IL-18 gene expression. In conclusion, thymol and thyme oil suppressed the expression of some inflammation-related genes and upregulated the expression of others. It can be stated that thymol and thyme oil may have positive effects on the prevention or correction of the inflammatory process from the gene level in the hepatic lipidosis model induced by HCD.Öğe Türü: Öğe , Detection of the presence of leptospirosis in horses by Elisa method(Afyon Kocatepe Üniversitesi, 03.10.2025) Karataş Yeni, Derya; Arslan, Duygu; Balevi, Aslı; Paksoy, YavuzkanLeptospirosis is one of the common zoonotic diseases worldwide. It significantly affects humans and animals, causing socio-economic losses. Equine leptospirosis often manifests itself with recurrent genital tract infections, reproductive disorders, abortion, embryonic death, and birth of weak foals. Studies on Leptospirosis, especially in horses, are quite limited. For this reason, our study was planned to investigate the transmission cycle in terms of farm animals and public health, and also to determine the presence of Leptospirosis in horses. For our research, 187 horse blood serum samples were collected from different settlements in Turkey. In the collected samples, Leptospira spp. ELISA test was used to investigate the presence of antibodies. A total of 8 samples (4.27%) were detected as seropositive in horse serum samples. The agent, a significant pathogen for both human and animal health, was identified as seropositive. In addition, the high rate of transmission of leptospirosis through contact with infected horses and the potential for spread through different animals should be taken into consideration. Leptospirosis was determined as seropositivity by ELISA in the horses sampled. Therefore, it is important to diagnose and control the infection rapidly.
