AKÜ Araştırma ve Akademik Performans Sistemi
DSpace@AKÜ, Afyon Kocatepe Üniversitesi’nin bilimsel araştırma ve akademik performansını izleme, analiz etme ve raporlama süreçlerini tek çatı altında buluşturan bütünleşik bilgi sistemidir.
Güncel Gönderiler
Öğe Türü: Öğe , Effect of chemical castration using high osmolarity solutions on spermatological parameters in rats(Afyon Kocatepe Üniversitesi, 03.10.2025) Şeyma Özer Kaya; İbrahim Canpolat; Hasan Akın [Please purchase a license if using PhraIn study, the changes in spermatological parameters in rats subjected to chemical castration with high osmolarity solutions were investigated. In study, 8 male rats were used in each group. No chemicals were applied to rats in control group. Rats in second group were sterilized surgically. 0.9% physiological saline was administered to 3rd group, 0.9% physiological saline and 10% calcium chloride to 4th group, 20% mannitol to 5th group, 20% mannitol and 10% calcium chloride to 6th group, 20% dextrose to 7th group, 20% dextrose and 10% calcium chloride solutions were administered intratesticularly to 8th group at dose of 0.1 ml/100gr. Andrological findings compared to control group; it was determined that there was a significant difference in sperm motility and sperm density (p<0.001) in groups 3, 5, 7. However, it was observed that there was no difference in rate of abnormal spermatozoon. In groups 2, 4, 6, 8, motility, density, abnormal sperm count and epididymis weights could not be measured because sperm cells could not be collected. In addition, it was determined that the weights of epididymis and right cauda epididymis in groups 3, 5, 7 decreased compared to control (p<0.05), while in groups 2, 4, 6, 8, measurements could not be made because tissue samples could not be taken, and weights of testicles, vesicle seminalis and prostate decreased significantly (p<0.001) compared to control. In conclusion; it has been concluded that giving high osmolarity solutions in combination with calcium chloride will be much more effective in intratesticular chemical sterilization process.Öğe Türü: Öğe , Explainable machine learning framework for milk quality grading(Afyon Kocatepe Üniversitesi, 03.10.2025) Çetintav, Bekir; Yalçın, AhmetThis study introduces an explainable machine learning framework for milk quality grading, combining high predictive performance with transparency and practicality. Utilizing Random Forest and HistGradientBoost models, alongside interpretability techniques like Permutation Feature Importance and LIME, the framework achieves robust classification while providing actionable insights. Global explanations identify pH and Temperature as critical factors, highlighting their significance in real-time monitoring and microbial control. Local explanations, based on the two presented examples, demonstrate the practical utility of individual predictions, offering targeted interventions such as optimizing storage conditions or addressing contamination risks. By bridging the gap between predictive accuracy and interpretability, this framework not only enhances trust and usability for stakeholders but also establishes a new perspective for integrating AI-driven quality control systems into the dairy industry.Öğe Türü: Öğe , Evaluation of postbiotics belonging to industrial kefir-derived exopolysaccharide-producing lacticaseibacillus rhamnosus and lacticaseibacillus paracasei as anti-candidal biofilm agents(Afyon Kocatepe Üniversitesi, 03.10.2025) Dışhan, Adalet; Abatay, Esmanur; Gönülalan, ZaferCandidal biofilm is a concern in both medical and industrial fields. This study aims to reveal the effect of postbiotics on the transcriptional level of biofilm-associated genes of Candida albicans in exopolysaccharide-producing industrial kefir-derived isolates and to determine their biofilm prevention and treatment properties. In the study, the exopolysaccharide production capacity of lactic acid bacteria obtained from kefir samples of eight different brands from supermarkets was determined. The transcriptional effect of postbiotics of exopolysaccharide-producing isolates on C. albicans biofilm-related genes was carried out via reverse transcription-polymerase chain reaction. The biofilm prevention and biofilm treatment effects of postbiotics were determined in abiotic media (polystyrene microplate). The minimum inhibitory concentration (MIC) values of Lacticaseibacillus rhamnosus and Lacticaseibacillus paracasei (exopolysaccharide-producing isolates) postbiotics were determined as 12.5% and 25%, respectively. While 2xMIC and above doses of L. rhamnosus and L. paracasei postbiotics were effective in prevention, MIC and above doses were effective in biofilm treatment. als1, als3, and bcr genes were down-regulated at 2xMIC and MIC doses of L. rhamnosus postbiotic. At exposure to a 2xMIC dose of L. paracasei postbiotic, all genes examined were down-regulated. On average, 0.03, 0.15, and 0.79-fold downregulation of als1, als3 and bcr genes was determined at 24 hours at 2xMIC dose of L. rhamnosus postbiotic; 0.31, 0.55, and 0.77-fold downregulation was determined at 24 hours at MIC doses. A 0.39, 0.05, 0.91, and 0.04-fold downregulation of L. paracasei postbiotic at 2xMIC dose at 24 hours was determined in als1, als3, bcr and hwp genes, respectively. In conclusion, the discovery of new approaches that can prevent or treat biofilms could stimulate the emergence of novel bio-control agents.Öğe Türü: Öğe , Spermatological and biochemical examination of New Zealand rabbit semen after thawing(Afyon Kocatepe Üniversitesi, 03.10.2025) Ozturk, Caner; Döşeyici, Mustafa Eren; Aksoy, Neşe HayatIn the present study, the effects of caffeic acid on rabbit semen were investigated using the antioxidant substance trehalose as a positive control. In the experiment, 4 male rabbits and 1 female rabbit were used. Ejaculates were collected via the artificial vaginal method 2 times a week for 4 weeks. Pooled semen samples were divided into four groups and diluted as follows: basic diluent+6%DMSO+caffeic acid (25µM and 50µM), basic diluent+6%DMSO+trehalose (positive control) (50mM), and basic diluent+6%DMSO (Control). After dilution, the semen samples were aspirated into straws and allowed to equilibrate at +4°C for 1 h. The samples were frozen in liquid nitrogen vapor and stored in liquid nitrogen. The highest value of sperm motility was detected in the 50 mM trehalose group (46.25±1.25%), which was significantly different from that in the control group (35.63±1.99%) (p<0.05). The sperm membrane integrity (SYBR 14/PI) results were determined to be 47.11±0.96%, 49.05±0.85%, and 49.79±1.04% in the 25 µM caffeic acid, 50µM caffeic acid, and 50 mM trehalose groups, respectively, and a significant difference was found between the supplement groups and the control group (39.01±1.21%) (p<0.05). In terms of acrosome integrity (FITC-PNA), the highest value was reached in the trehalose 50 mM group (48.70±1.03%), and a significant difference was observed compared with that in the control group (41.48±0.80%). A significant difference was observed in the total antioxidant capacity and total oxidant level values between the 50 mM trehalose group and the control group (p<0.05). As a result, caffeic acid added to the extender when rabbit semen was frozen had a better protective effect at higher doses.Öğe Türü: Öğe , Evaluation of tumor necrosis factor-alfa, cholesterol, bhba, nefa and acetylcholinesterase levels in cows with ketosis(Afyon Kocatepe Üniversitesi, 03.10.2025) Yenilmez, Yücel; Aytekin, İsmailIn this study serum beta-hydroxybutyric acid, glucose, total protein, triglyceride, cholesterol, non-esterified fatty acids, acetylcholinesterase and tumor necrosis factor-α were investigated in cows with ketosis. The study material consisted of 10 control and 10 group with ketosis, total 20 holstein dairy cows of 10 control and 10 groups with ketosis, a total of 20 holstein dairy cows aged between 3 and 7 years. In this study serum concentration was measured for nonesterified fatty acids, beta-hydroxybutyric acid, aspartate aminotransferase, total protein, cholesterol, triglyceride, acetylcholinesterase and tumor necrosis factor-α in the healthy group and cows with ketosis. Serum glucose, triglyceride, nonesterified fatty acids, acetylcholinesterase and tumor necrosis factor-α did not differ significantly between the two groups. Aspartate aminotransferase and beta-hydroxybutyric acid increased, while cholesterol and total protein concentration decreased in ketotic cows compared with healthy cows. Consequently, acetylcholinesterase and tumor necrosis factor-α concentrations may prove beneficial biochemical findings in cows with ketosis.
