Effects of supplementation of Poopy Seed and Poopy Seed Oil at Various Quantities on Oxidant-antioxidant Balance in Laying Hens

Abstract

This study was conducted to determine the effect of rations containing different amounts of Poppy seed (PS) and Poppy seed oil (PSO) on oxidant-antioxidant balance in laying hens. There was a control (unsupplemented) and six experimental groups (supplemented with 0.5,1.0 and 1.5 % PS and 0.5,1.0 and 1.5, % PSO ). Totally 360 and 24 weeks old laying hens were utilized. The experiment was lasted for 8 weeks. The blood malondialdehyde (MDA), nitric oxide (NOx), vitamin C, -carotene and vitamin A and levels, between PS groups did not differ. The reduced glutathione (GSH) levels in 1.0% and 1.5 % PS groups increased significantly compared to controls group. The MDA level was found to be significantly lower in the 0.5 % PSO group than in the controls and other PSO groups. The GSH levels in 0,5 % and 1.0% PS groups increased significantly compared to controls group. The NOx levels in 0,5% PSO group significantly decreased compared to other experimental groups. The Vitamin A levels in 0,5%, 1,0% and 1,5 PSO groups increased significantly compared to control group. The vitamin C and carotene levels between PSO groups did not differ. According to the findings of this study, the 0.5% supplementation of PSO to the diets of laying hens decrease the blood MDA levels and increase the GSH and vitamin A concentrations. It is thought that 0.5% PSO supplementation can be used effectively as an antioxidant supplement in laying hens.